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J Am Chem Soc. 2009 Mar 11;131(9):3225-9. doi: 10.1021/ja806752h.

Biosynthesis of the thiamin thiazole in Bacillus subtilis: identification of the product of the thiazole synthase-catalyzed reaction.

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  • 1Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14853, USA.

Abstract

In this paper, we describe an optimized reconstitution of the thiamin thiazole synthase (ThiG) catalyzed reaction and demonstrate that the enzymatic product is an unanticipated dearomatized thiazole tautomer.

PMID:
19216519
[PubMed - indexed for MEDLINE]
PMCID:
PMC2765510
Free PMC Article
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