Ion-transfer voltammetric determination of the beta-blocker propranolol in a physiological matrix at silicon membrane-based liquid|liquid microinterface arrays

Anal Chem. 2009 Mar 15;81(6):2344-9. doi: 10.1021/ac802644g.

Abstract

In this work, the ion-transfer voltammetric detection of the protonated beta-blocker propranolol in artificial saliva is presented. Cyclic voltammetry, differential pulse voltammetry, and differential pulse stripping voltammetry (DPSV) were employed in the detection of the cationic drug based on ion-transfer voltammetry across arrays of microinterfaces between artificial saliva and an organogel phase. It was found that the artificial saliva matrix decreased the available potential window for ion-transfer voltammetry at this liquid|liquid interface but transfer of protonated propranolol was still achieved. The DPSV method employed a preconditioning step as well as a preconcentration step followed by analytical signal generation based on the back-transfer of the drug across the array of microinterfaces. The DPSV peak current response was linear with drug concentration in the artificial saliva matrix over the concentration range of 0.05-1 microM (i(p) = -8.13 (nA microM(-1))(concentration) + 0.07 (nA), R = 0.9929, n = 7), and the calculated detection limit (3s(b)) was 0.02 microM. These results demonstrate that DPSV at arrays of liquid|liquid microinterfaces is a viable analytical approach for pharmaceutical determinations in biomimetic matrixes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenergic beta-Antagonists / analysis*
  • Electrochemical Techniques / instrumentation
  • Electrochemical Techniques / methods*
  • Ion Transport
  • Propranolol / analysis*
  • Silicon / chemistry*

Substances

  • Adrenergic beta-Antagonists
  • Propranolol
  • Silicon