Shown is the combined nucleosome fold depletion over all poly(dA:dT) tracts of length k, for k = 5,6,7,…, and for tracts with exactly 0, 2, 4, or 6 base substitutions. Each graph is trimmed at a length K at which there are less than 10 such tracts in the S. cerevisiae genome, and the fold depletion at this final point is computed over all elements whose length is at least K. The number of underlying elements at various points in the graph is indicated (N). Figure adapted from ref. [**9].

Shown is a representation of the atomic resolution X-ray crystallographic structure of [d(CGCAAATTTGCG)]₂ [**44]. The DNA backbones are shown as yellow curves, with the bases shown in a partial-charge-coded stick representation. The narrow minor groove of the A₃T₃ stretch has many high occupancy water molecules, 4 layers deep, shown here as spheres, color coded according to their layer from innermost to outermost as cyan, purple, blue, and red, respectively. The multiple layers, extensive hydrogen bonding, and high occupancy of these waters all suggest that they may have strongly favorable energetic interactions with themselves and the DNA. Figure kindly provided by Prof. L.D. Williams (Georgia Tech.).

(a) Shown is a simple example focusing only on the immediate neighborhood of the boundary. All (five) possible nucleosome configurations are illustrated, in which a nucleosome (cyan ovals) can be placed within five basepairs of the boundary (blue triangle). The number and set of nucleosome configurations occupying each of the five basepairs immediately adjacent to the boundary are shown in the graph below. If all configurations are equally likely, then basepairs closer to the poly(dA:dT) tract will exhibit lower nucleosome occupancy simply because fewer nucleosome configurations cover those basepairs [22]. (b) Schematic showing that nucleosome depletion caused by a poly(dA:dT) tract is maximal over the tract itself, but extends for considerable distances in either direction. Thus specific factor DNA binding sites located nearby to a poly(dA:dT) tract will have relatively enhanced accessibility compared to factor sites located far from a poly(dA:dT) tract, facilitating binding of the factor. Panel (a) adapted from ref. [**9].