Display Settings:

Format

Send to:

Choose Destination
Biotechnol Lett. 2009 Jun;31(6):819-23. doi: 10.1007/s10529-009-9940-5. Epub 2009 Feb 11.

Validity of DNA analysis to determine cell numbers in tissue engineering scaffolds.

Author information

  • 1Centre for Regenerative Medicine, Department of Chemical Engineering, University of Bath, Bath BA2 7AY, UK.

Abstract

The potential of a DNA content assay, PicoGreen, for use in 3D bioengineered constructs was examined. The assay was tested on ATDC5 cells in situ during culture in typical tissue engineering 3D constructs. Comparisons of cell standards from cell lines and primary cells to lambdaDNA standards was also conducted. An effective working range of the assay within 3D constructs was shown up to 2.5 x 10(5) cells ml(-1). From significant variation found in DNA content between cell lines and primary cells, it was concluded that the most accurate standard to use for the assay was from the cell type being examined.

PMID:
19205891
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Springer
    Loading ...
    Write to the Help Desk