Sortase-mediated ligation: a gift from Gram-positive bacteria to protein engineering

Chembiochem. 2009 Mar 23;10(5):787-98. doi: 10.1002/cbic.200800724.

Authors

Shinya Tsukiji¹, Teruyuki Nagamune

Affiliation

¹ Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.

PMID: 19199328
DOI: 10.1002/cbic.200800724

No abstract available

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Amino Acid Sequence
Aminoacyltransferases / genetics
Aminoacyltransferases / metabolism*
Animals
Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
Cell Line
Cysteine Endopeptidases / genetics
Cysteine Endopeptidases / metabolism*
Gram-Positive Bacteria / metabolism*
Humans
Models, Molecular
Molecular Sequence Data
Molecular Structure
Nucleic Acids / chemistry
Peptidoglycan / metabolism
Protein Engineering / methods*
Protein Splicing*
Protein Structure, Tertiary
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / isolation & purification
Recombinant Fusion Proteins / metabolism
Substrate Specificity

Substances

Bacterial Proteins
Nucleic Acids
Peptidoglycan
Recombinant Fusion Proteins
Aminoacyltransferases
Cysteine Endopeptidases