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Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2009 Feb;26(1):45-9. doi: 10.3760/cma.j.issn.1003-9406.2009.01.010.

[Multiplex ligation-dependent probe amplification and its application].

[Article in Chinese]

Author information

  • 1Shanghai Institute of Medical Genetics, Shanghai Jiaotong University, Shanghai, 200040 PR China.


Multiplex ligation-dependent probe amplification (MLPA) is a semiquantitative analysis based on polymerase chain reaction (PCR). It possesses many advantages such as high efficiency, simple operation, low cost and has been wildly applied in researches of diseases associated with copy number variation, point mutation and methylation. Recently, MLPA is combined with DNA chip to become a real high-throughput method and get great improvement in reliability. Here, the progresses of methods and application of MLPA, as well as its limitations are reviewed.

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