Trans R Soc Trop Med Hyg. 2009 Apr;103(4):342-6. Epub 2009 Feb 4.

Molecular diagnosis of Strongyloides stercoralis in faecal samples using real-time PCR.

Verweij JJ, Canales M, Polman K, Ziem J, Brienen EA, Polderman AM, van Lieshout L.

Source

Leiden University Medical Center, Department of Parasitology, Postbus 9600, 2300 RC Leiden, The Netherlands. j.j.verweij@lumc.nl

Erratum in

Trans R Soc Trop Med Hyg. 2010 May;104(5):378.

Abstract

A real-time PCR method targeting the small subunit of the rRNA gene was developed for the detection of Strongyloides stercoralis DNA in faecal samples, including an internal control to detect inhibition of the amplification process. The assay was performed on a range of well-defined control samples (n=145), known positive faecal samples (n=38) and faecal samples from a region in northern Ghana where S. stercoralis infections are highly endemic (n=212), and achieved 100% specificity and high sensitivity. The use of this assay could facilitate monitoring the prevalence and intensity of S. stercoralis infections during helminth intervention programs. Moreover, the use of this assay in diagnostic laboratories could make the introduction of molecular diagnostics feasible in the routine diagnosis of S. stercoralis infections, with a two-fold increase in the detection rate as compared with the commonly used Baermann sedimentation method.

PMID:: 19195671; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

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Research Support, Non-U.S. Gov't

MeSH Terms

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DNA, Protozoan

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