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Methods Enzymol. 2008;451:151-62. doi: 10.1016/S0076-6879(08)03211-4.

Cell-free reconstitution of microautophagy in yeast.

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  • 1D├ępartement de Biochimie, Chemin des Boveresses 155, CH-1066 Epalinges, Switzerland.

Abstract

Microautophagy is the direct uptake of soluble or particulate cellular constituents into lysosomes. Here, I describe methods to reconstitute and study this process in vitro, using vacuoles (lysosomes) from the yeast Saccharomyces cerevisiae as model organelles. Protocols to grow the cells, isolate vacuoles from them, and to induce microautophagy of soluble tracers are presented.

PMID:
19185719
[PubMed - indexed for MEDLINE]
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