Science. 2009 Mar 20;323(5921):1600-4. Epub 2009 Jan 29.

A role for RNAi in the selective correction of DNA methylation defects.

Teixeira FK, Heredia F, Sarazin A, Roudier F, Boccara M, Ciaudo C, Cruaud C, Poulain J, Berdasco M, Fraga MF, Voinnet O, Wincker P, Esteller M, Colot V.

Source

Unité de Recherche en Génomique Végétale, CNRS UMR 8114, Institut National de la Recherche Argonomique UMR 1165, Université d'Evry Val d'Essonne, 91057 Evry Cedex, France.

Abstract

DNA methylation is essential for silencing transposable elements and some genes in higher eukaryotes, which suggests that this modification must be tightly controlled. However, accidental changes in DNA methylation can be transmitted through mitosis (as in cancer) or meiosis, leading to epiallelic variation. We demonstrated the existence of an efficient mechanism that protects against transgenerational loss of DNA methylation in Arabidopsis. Remethylation is specific to the subset of heavily methylated repeats that are targeted by the RNA interference (RNAi) machinery. This process does not spread into flanking regions, is usually progressive over several generations, and faithfully restores wild-type methylation over target sequences in an RNAi-dependent manner. Our findings suggest an important role for RNAi in protecting genomes against long-term epigenetic defects.

Comment in

Science. 2009 Mar 20;323(5921):1568-9.

PMID:: 19179494; [PubMed - indexed for MEDLINE]

Free full text

Publication Types, MeSH Terms, Substances, Secondary Source ID

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

Secondary Source ID

GEO/GSE13419

LinkOut - more resources

Full Text Sources

Other Literature Sources

COS Scholar Universe

Supplemental Content

Save items

Related citations in PubMed

RNAi of met1 reduces DNA methylation and induces genome-specific changes in gene expression and centromeric small RNA accumulation in Arabidopsis allopolyploids. [Genetics. 2008]
RNAi of met1 reduces DNA methylation and induces genome-specific changes in gene expression and centromeric small RNA accumulation in Arabidopsis allopolyploids.
Chen M, Ha M, Lackey E, Wang J, Chen ZJ. Genetics. 2008 Apr; 178(4):1845-58.
Molecular biology. Dynamic DNA methylation. [Science. 2009]
Molecular biology. Dynamic DNA methylation.
Law JA, Jacobsen SE. Science. 2009 Mar 20; 323(5921):1568-9.
Genome-wide demethylation of Arabidopsis endosperm. [Science. 2009]
Genome-wide demethylation of Arabidopsis endosperm.
Hsieh TF, Ibarra CA, Silva P, Zemach A, Eshed-Williams L, Fischer RL, Zilberman D. Science. 2009 Jun 12; 324(5933):1451-4.
Review RNA-directed DNA methylation and Pol IVb in Arabidopsis. [Cold Spring Harb Symp Quant Bi...]
Review RNA-directed DNA methylation and Pol IVb in Arabidopsis.
Matzke M, Kanno T, Huettel B, Daxinger L, Matzke AJ. Cold Spring Harb Symp Quant Biol. 2006; 71:449-59.
Review DNA methylation dynamics in plant genomes. [Biochim Biophys Acta. 2007]
Review DNA methylation dynamics in plant genomes.
Gehring M, Henikoff S. Biochim Biophys Acta. 2007 May-Jun; 1769(5-6):276-86. Epub 2007 Feb 7.

See reviews... See all...

Cited by 33 PubMed Central articles

The impact of the organism on its descendants. [Genet Res Int. 2012]
The impact of the organism on its descendants.
Bateson P. Genet Res Int. 2012; 2012:640612. Epub 2011 Dec 1.
Stress-induced chromatin changes: a critical view on their heritability. [Plant Cell Physiol. 2012]
Stress-induced chromatin changes: a critical view on their heritability.
Pecinka A, Mittelsten Scheid O. Plant Cell Physiol. 2012 May; 53(5):801-8. Epub 2012 Mar 28.
Loss of DNA methylation affects the recombination landscape in Arabidopsis. [Proc Natl Acad Sci U S A. 2012]
Loss of DNA methylation affects the recombination landscape in Arabidopsis.
Mirouze M, Lieberman-Lazarovich M, Aversano R, Bucher E, Nicolet J, Reinders J, Paszkowski J. Proc Natl Acad Sci U S A. 2012 Apr 10; 109(15):5880-5. Epub 2012 Mar 26.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
GEO DataSets
Gene expression and molecular abundance data reported in the current articles that are also included in the curated Gene Expression Omnibus (GEO) DataSets.
Related Project
Related Projects
SRA
Massively-parallel sequencing project data in the Short Read Archive (SRA) that are reported in the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

A role for RNAi in the selective correction of DNA methylation defects.
A role for RNAi in the selective correction of DNA methylation defects.
Science. 2009 Mar 20 ;323(5921):1600-4. Epub 2009 Jan 29 .

PubMed
Biologic lung volume reduction in advanced upper lobe emphysema: phase 2 results...
Biologic lung volume reduction in advanced upper lobe emphysema: phase 2 results.
Am J Respir Crit Care Med. 2009 May 1 ;179(9):791-8. Epub 2009 Jan 29 .

PubMed
Minireview: Pathophysiological importance of thyroid hormone transporters.
Minireview: Pathophysiological importance of thyroid hormone transporters.
Endocrinology. 2009 Mar ;150(3):1078-83. Epub 2009 Jan 29 .

PubMed
Negative regulation of cell proliferation by mevalonate or one of the mevalonate...
Negative regulation of cell proliferation by mevalonate or one of the mevalonate phosphates.
J Biol Chem. 1991 Sep 25 ;266(27):17966-71.

PubMed
Transgenerational effect of neighborhood poverty on low birth weight among Afric...
Transgenerational effect of neighborhood poverty on low birth weight among African Americans in Cook County, Illinois.
Am J Epidemiol. 2009 Mar 15 ;169(6):712-7. Epub 2009 Jan 29 .

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: