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PLoS One. 2009;4(1):e4304. doi: 10.1371/journal.pone.0004304. Epub 2009 Jan 28.

A role for the juxtamembrane cytoplasm in the molecular dynamics of focal adhesions.

Author information

  • 1Department of Neurobiology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv, Israel.

Erratum in

  • PLoS ONE. 2009;4(3). doi: 10.1371/annotation/ce274a43-a284-4839-9791-59e4b2563c9c.

Abstract

Focal adhesions (FAs) are specialized membrane-associated multi-protein complexes that link the cell to the extracellular matrix and play crucial roles in cell-matrix sensing. Considerable information is available on the complex molecular composition of these sites, yet the regulation of FA dynamics is largely unknown. Based on a combination of FRAP studies in live cells, with in silico simulations and mathematical modeling, we show that the FA plaque proteins paxillin and vinculin exist in four dynamic states: an immobile FA-bound fraction, an FA-associated fraction undergoing exchange, a juxtamembrane fraction experiencing attenuated diffusion, and a fast-diffusing cytoplasmic pool. The juxtamembrane region surrounding FAs displays a gradient of FA plaque proteins with respect to both concentration and dynamics. Based on these findings, we propose a new model for the regulation of FA dynamics in which this juxtamembrane domain acts as an intermediary layer, enabling an efficient regulation of FA formation and reorganization.

PMID:
19172999
[PubMed - indexed for MEDLINE]
PMCID:
PMC2627934
Free PMC Article
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