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    FEBS Lett. 2009 Feb 18;583(4):703-10. Epub 2009 Jan 21.

    Cell surface sialylation and fucosylation are regulated by the cell recognition molecule L1 via PLCgamma and cooperate to modulate embryonic stem cell survival and proliferation.

    Li YL, Wu GZ, Zeng L, Dawe GS, Sun L, Loers G, Tilling T, Cui SS, Schachner M, Xiao ZC.

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    Institute of Molecular and Cellular Medicine, Kunming Medical College, China.

    Abstract

    Cell surface glycosylation patterns are markers of cell type and status. However, the mechanisms regulating surface glycosylation patterns remain unknown. Using a panel of carbohydrate markers, we have shown that cell surface sialylation and fucosylation are upregulated in L1-transfected embryonic stem cells (L1-ESCs). Consistently, the mRNA levels of sialyltransferase ST6Gal1 and ST3Gal4, and fucosyltransferase FUT9 were significantly increased in L1-transfected ESCs. Activation of L1 signaling promoted cell survival and inhibited cell proliferation. ShRNAs knocking down FUT9, ST6Gal1 and ST3Gal4 blocked these effects. A phospholipase Cgamma (PLCgamma) inhibitor and shRNA reduced ST6Gal1, ST3Gal4 and FUT9 mRNA levels in the L1-ESCs. Thus, embryonic stem cell surface sialylation and fucosylation are regulated via PLCgamma by L1, with which they cooperate to modulate cell survival and proliferation.

    PMID:
    19166842
    [PubMed - indexed for MEDLINE]

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      Cited by 1 PubMed Central article

      • The N-glycome of human embryonic stem cells. [BMC Cell Biol. 2009]
        The N-glycome of human embryonic stem cells.
        Satomaa T, Heiskanen A, Mikkola M, Olsson C, Blomqvist M, Tiittanen M, Jaatinen T, Aitio O, Olonen A, Helin J, et al. BMC Cell Biol. 2009 Jun 2; 10:42. Epub 2009 Jun 2.

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