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    Proc Natl Acad Sci U S A. 2009 Feb 17;106(7):2159-64. Epub 2009 Jan 21.

    Electron microscopy of whole cells in liquid with nanometer resolution.

    de Jonge N, Peckys DB, Kremers GJ, Piston DW.

    Source

    Department of Molecular Physiology and Biophysics, Vanderbilt University Medical Center, Nashville, TN 37232-0615, USA. niels.de.jonge@vanderbilt.edu

    Abstract

    Single gold-tagged epidermal growth factor (EGF) molecules bound to cellular EGF receptors of fixed fibroblast cells were imaged in liquid with a scanning transmission electron microscope (STEM). The cells were placed in buffer solution in a microfluidic device with electron transparent windows inside the vacuum of the electron microscope. A spatial resolution of 4 nm and a pixel dwell time of 20 micros were obtained. The liquid layer was sufficiently thick to contain the cells with a thickness of 7 +/- 1 microm. The experimental findings are consistent with a theoretical calculation. Liquid STEM is a unique approach for imaging single molecules in whole cells with significantly improved resolution and imaging speed over existing methods.

    PMID:
    19164524
    [PubMed - indexed for MEDLINE]
    PMCID:
    PMC2650183
    Free PMC Article

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