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Mol Cell Biol. 2009 Apr;29(7):1707-18. doi: 10.1128/MCB.01184-07. Epub 2009 Jan 21.

Key role for activin B in cellular transformation after loss of the von Hippel-Lindau tumor suppressor.

Author information

  • 1Nikolaus Fiebiger Center, University Erlangen-Nürnberg, Glückstr. 6, 91054 Erlangen, Germany.

Abstract

The von Hippel-Lindau tumor suppressor gene (VHL) is mutated in clear cell renal cell carcinomas (RCC), leading to the activation of hypoxia-inducible factor (HIF)-mediated gene transcription. Several VHL/HIF targets, such as glycolysis, angiogenesis, cell growth, and chemotaxis of tumor cells, have been implicated in the transformed phenotype of RCC-regulating properties. Here, we show that VHL suppresses key features of cell transformation through downregulation of the HIF-dependent expression of activin B, a member of the transforming growth factor beta superfamily. Activin B expression is repressed by restoration of VHL in VHL-deficient RCC cells and upregulated by hypoxia. RCC tumor samples show increased expression of activin B compared to that in the normal kidney. VHL increases cell adhesion to the extracellular matrix, promotes cell flattening, and reduces invasiveness. These effects are completely phenocopied by RNA interference-mediated knockdown of activin B and reverted by treatment with recombinant activin B. Finally, knockdown of activin B reduces tumor growth of RCC cells in nude mice. Our data indicate that activin B is a key mediator of VHL/HIF-induced transformation in RCC.

PMID:
19158274
[PubMed - indexed for MEDLINE]
PMCID:
PMC2655617
Free PMC Article

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