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J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Feb 15;877(5-6):497-501. doi: 10.1016/j.jchromb.2008.12.051. Epub 2008 Dec 27.

Quantitative analysis of RU38486 (mifepristone) by HPLC triple quadrupole mass spectrometry.

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  • 1Mass Spectrometry Core Laboratory and Centre for Cardiovascular Sciences, Wellcome Trust Clinical Research Facility, Queen's Medical Research Institute, University of Edinburgh, 47 Little France Crescent, Edinburgh EH16 4TJ, United Kingdom.


A sensitive liquid chromatography-mass spectrometric method was validated for the quantification of RU38486 (mifepristone) in human and murine plasma. The analyte and internal standard (alfaxolone) were extracted by liquid-liquid extraction with diethyl ether, resolved on a C18 column using gradient elution with methanol and ammonium acetate and detected after positive electrospray ionization (m/z 430-->372; m/z 333-->297, respectively). Quantification was linear over the range 0.5-500ng (r(2)>0.997), precise and accurate (intra-assay RSD< or =7.2%, RME< or =8.2%; inter-assay RSD< or =15.7% RME< or =10.2%). The limit of quantification (LOQ) was 50pg injected on column, permitting reproducible analysis of RU38486 in small volumes of plasma.

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