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J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Feb 15;877(5-6):497-501. doi: 10.1016/j.jchromb.2008.12.051. Epub 2008 Dec 27.

Quantitative analysis of RU38486 (mifepristone) by HPLC triple quadrupole mass spectrometry.

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  • 1Mass Spectrometry Core Laboratory and Centre for Cardiovascular Sciences, Wellcome Trust Clinical Research Facility, Queen's Medical Research Institute, University of Edinburgh, 47 Little France Crescent, Edinburgh EH16 4TJ, United Kingdom.

Abstract

A sensitive liquid chromatography-mass spectrometric method was validated for the quantification of RU38486 (mifepristone) in human and murine plasma. The analyte and internal standard (alfaxolone) were extracted by liquid-liquid extraction with diethyl ether, resolved on a C18 column using gradient elution with methanol and ammonium acetate and detected after positive electrospray ionization (m/z 430-->372; m/z 333-->297, respectively). Quantification was linear over the range 0.5-500ng (r(2)>0.997), precise and accurate (intra-assay RSD< or =7.2%, RME< or =8.2%; inter-assay RSD< or =15.7% RME< or =10.2%). The limit of quantification (LOQ) was 50pg injected on column, permitting reproducible analysis of RU38486 in small volumes of plasma.

PMID:
19157997
[PubMed - indexed for MEDLINE]
PMCID:
PMC4392796
Free PMC Article
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