Bioorg Med Chem Lett. 2009 Feb 15;19(4):1258-60. Epub 2008 Dec 27.

A rapid method for generation of selective Sox-based chemosensors of Ser/Thr kinases using combinatorial peptide libraries.

González-Vera JA, Luković E, Imperiali B.

Source

Department of Chemistry, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.

Abstract

A novel screening method to identify selective Sox-based fluorescent probes for Ser/Thr kinases has been developed. Peptide libraries were exposed to a kinase of interest and the products of the timed reaction were analyzed by MALDI-TOF. To demonstrate the potential of this methodology, a selective substrate for Aurora A kinase was identified that showed a 7-fold improvement in catalytic efficiency over the best substrate described to date in the literature.

PMID:: 19157873; [PubMed - indexed for MEDLINE]
PMCID:: PMC2838374

Free PMC Article

Images from this publication.See all images (2) Free text

Publication Types, MeSH Terms, Substances, Grant Support

Publication Types

MeSH Terms

Substances

Grant Support

LinkOut - more resources

Full Text Sources

Other Literature Sources

COS Scholar Universe

Supplemental Content

Save items

Related citations in PubMed

Method for screening and MALDI-TOF MS sequencing of encoded combinatorial libraries. [Anal Chem. 2007]
Method for screening and MALDI-TOF MS sequencing of encoded combinatorial libraries.
Hu BH, Jones MR, Messersmith PB. Anal Chem. 2007 Oct 1; 79(19):7275-85. Epub 2007 Aug 23.
High-throughput one-bead-one-compound approach to peptide-encoded combinatorial libraries: MALDI-MS analysis of single TentaGel beads. [J Comb Chem. 2003]
High-throughput one-bead-one-compound approach to peptide-encoded combinatorial libraries: MALDI-MS analysis of single TentaGel beads.
Franz AH, Liu R, Song A, Lam KS, Lebrilla CB. J Comb Chem. 2003 Mar-Apr; 5(2):125-37.
Review "Whole cell"--matrix-assisted laser desorption ionization-time of flight-mass spectrometry, an emerging technique for efficient screening of biocombinatorial libraries of natural compounds-present state of research. [Comb Chem High Throughput Scre...]
Review "Whole cell"--matrix-assisted laser desorption ionization-time of flight-mass spectrometry, an emerging technique for efficient screening of biocombinatorial libraries of natural compounds-present state of research.
Vater J, Gao X, Hitzeroth G, Wilde C, Franke P. Comb Chem High Throughput Screen. 2003 Sep; 6(6):557-67.
Development of high-throughput phosphorylation profiling method for identification of Ser/Thr kinase specificity. [J Pept Sci. 2011]
Development of high-throughput phosphorylation profiling method for identification of Ser/Thr kinase specificity.
Kim EM, Kim J, Kim YG, Lee P, Shin DS, Kim M, Hahn JS, Lee YS, Kim BG. J Pept Sci. 2011 May; 17(5):392-7. Epub 2010 Nov 22.
Review Substrate screening of protein kinases: detection methods and combinatorial peptide libraries. [Biopolymers. 2010]
Review Substrate screening of protein kinases: detection methods and combinatorial peptide libraries.
Kim M, Shin DS, Kim J, Lee YS. Biopolymers. 2010; 94(6):753-62.

See reviews... See all...

Cited by 2 PubMed Central articles

A p38α-selective chemosensor for use in unfractionated cell lysates. [ACS Chem Biol. 2011]
A p38α-selective chemosensor for use in unfractionated cell lysates.
Stains CI, Luković E, Imperiali B. ACS Chem Biol. 2011 Jan 21; 6(1):101-5. Epub 2010 Sep 30.
Synthesis of red-shifted 8-hydroxyquinoline derivatives using click chemistry and their incorporation into phosphorylation chemosensors. [J Org Chem. 2009]
Synthesis of red-shifted 8-hydroxyquinoline derivatives using click chemistry and their incorporation into phosphorylation chemosensors.
González-Vera JA, Luković E, Imperiali B. J Org Chem. 2009 Oct 2; 74(19):7309-14.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
BioAssay
PubChem BioAssay experiments on the biological activities of small molecules that cite the current articles. The depositors of BioAssay data provide these references.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

A rapid method for generation of selective Sox-based chemosensors of Ser/Thr kin...
A rapid method for generation of selective Sox-based chemosensors of Ser/Thr kinases using combinatorial peptide libraries.
Bioorg Med Chem Lett. 2009 Feb 15 ;19(4):1258-60. Epub 2008 Dec 27 .

PubMed
Oligomerized grape seed polyphenols attenuate inflammatory changes due to antiox...
Oligomerized grape seed polyphenols attenuate inflammatory changes due to antioxidative properties in coculture of adipocytes and macrophages.
J Nutr Biochem. 2010 Jan ;21(1):47-54. Epub 2009 Jan 20 .

PubMed
A potential proliferative gene, NUDT6, is down-regulated by green tea catechins ...
A potential proliferative gene, NUDT6, is down-regulated by green tea catechins at the posttranscriptional level.
J Nutr Biochem. 2010 Feb ;21(2):98-106. Epub 2009 Jan 20 .

PubMed
Green tea inhibits Helicobacter growth in vivo and in vitro.
Green tea inhibits Helicobacter growth in vivo and in vitro.
Int J Antimicrob Agents. 2009 May ;33(5):473-8. Epub 2009 Jan 20 .

PubMed
[Feasibility of out-of-hospital management of non-heart-beating donors in Seine-...
[Feasibility of out-of-hospital management of non-heart-beating donors in Seine-Saint-Denis: one year retrospective study].
Ann Fr Anesth Reanim. 2009 Feb ;28(2):124-9. Epub 2009 Jan 20 .

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: