A novel assay for the in vivo study of Schwann cells

Exp Neurol. 1991 Oct;114(1):140-3. doi: 10.1016/0014-4886(91)90092-q.

Abstract

An in vivo assay was developed for long-term analyses of Schwann cells at the single cell level. Schwann cells were isolated from sciatic nerves and labeled with the fluorescent gold label Fluoro-Gold. Cells were then transplanted into severed sciatic nerves of young adult rats. Gold-labeled Schwann cells, identified by double-labeling with S100 antibodies, were observed up to 10 mm away from the transplant site and after 90 and 120 days of survival in vivo.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies
  • Cell Movement
  • Cell Separation
  • Cell Survival
  • Cytological Techniques*
  • Fluorescent Dyes
  • S100 Proteins / immunology
  • Schwann Cells / physiology*
  • Schwann Cells / transplantation
  • Sciatic Nerve / cytology
  • Sciatic Nerve / physiology
  • Stilbamidines*
  • Time Factors

Substances

  • 2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt
  • Antibodies
  • Fluorescent Dyes
  • S100 Proteins
  • Stilbamidines