From outside to inside, the first two circles represent the COG assignment for predicted coding sequences on the plus and minus strands, respectively. Colors indicate the following: dark gray, hypothetical proteins; light gray, conserved hypothetical and unknown function; brown, general function prediction; red, replication and repair; green, energy metabolism; blue, carbon and carbohydrate metabolism; cyan, lipid metabolism; magenta, transcription; yellow, translation; orange, amino acid metabolism; pink, metabolism of cofactors and vitamins; light red, purine and pyrimidine metabolism; lavender, signal transduction; sky blue, cellular processes. The third circle represents the RNA genes, red for rRNA and green for tRNA genes. The forth circle corresponds to genes with better Blast hits to genes outside Firmicutes and the fifth circle (red lines) to genes without homologs among the Clostridia. The two innermost circles represent the percent G+C content and G+C skew values, respectively.