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Genome Res. 2009 Apr;19(4):533-44. doi: 10.1101/gr.086496.108. Epub 2009 Jan 13.

Human gamma-satellite DNA maintains open chromatin structure and protects a transgene from epigenetic silencing.

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  • 1Laboratory of Molecular Pharmacology, National Cancer Institute, Bethesda, Maryland 20892, USA.

Abstract

The role of repetitive DNA sequences in pericentromeric regions with respect to kinetochore/heterochromatin structure and function is poorly understood. Here, we use a mouse erythroleukemia cell (MEL) system for studying how repetitive DNA assumes or is assembled into different chromatin structures. We show that human gamma-satellite DNA arrays allow a transcriptionally permissive chromatin conformation in an adjacent transgene and efficiently protect it from epigenetic silencing. These arrays contain CTCF and Ikaros binding sites. In MEL cells, this gamma-satellite DNA activity depends on binding of Ikaros proteins involved in differentiation along the hematopoietic pathway. Given our discovery of gamma-satellite DNA in pericentromeric regions of most human chromosomes and a dynamic chromatin state of gamma-satellite arrays in their natural location, we suggest that gamma-satellite DNA represents a unique region of the functional centromere with a possible role in preventing heterochromatin spreading beyond the pericentromeric region.

PMID:
19141594
[PubMed - indexed for MEDLINE]
PMCID:
PMC2665773
Free PMC Article

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