Display Settings:

Format

Send to:

Choose Destination
Nat Methods. 2009 Jan;6(1):24-32. doi: 10.1038/nmeth.1291.

Microscopy and its focal switch.

Author information

  • Max Planck Institute for Biophysical Chemistry, Department of NanoBiophotonics, 37070 Göttingen, Germany. hell@nanoscopy.de

Abstract

Until not very long ago, it was widely accepted that lens-based (far-field) optical microscopes cannot visualize details much finer than about half the wavelength of light. The advent of viable physical concepts for overcoming the limiting role of diffraction in the early 1990s set off a quest that has led to readily applicable and widely accessible fluorescence microscopes with nanoscale spatial resolution. Here I discuss the principles of these methods together with their differences in implementation and operation. Finally, I outline potential developments.

PMID:
19116611
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group
    Loading ...
    Write to the Help Desk