Warning: The NCBI web site requires JavaScript to function. more...
Generate a file for use with external citation management software.
Center for Research at the Bio/Nano Interface, Department of Chemistry, UF Genetics Institute, and McKnight Brain Institute, University of Florida, Gainesville, Florida 32611-7200, USA.
This work describes the development and investigation of an aptamer modified microfluidic device that captures rare cells to achieve a rapid assay without pretreatment of cells. To accomplish this, aptamers are first immobilized on the surface of a poly(dimethylsiloxane) microchannel, followed by pumping a mixture of cells through the device. This process permits the use of optical microscopy to measure the cell-surface density from which we calculate the percentage of cells captured as a function of cell and aptamer concentration, flow velocity, and incubation time. This aptamer-based device was demonstrated to capture target cells with >97% purity and >80% efficiency. Since the cell capture assay is completed within minutes and requires no pretreatment of cells, the device promises to play a key role in the early detection and diagnosis of cancer where rare diseased cells can first be enriched and then captured for detection.
Images from this publication.See all images (4) Free text
Your browsing activity is empty.
Activity recording is turned off.
Turn recording back on