Int J Legal Med. 1991;104(3):145-8.

Extraction strategy for obtaining DNA from bloodstains for PCR amplification and typing of the HLA-DQ alpha gene.

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Forensic Science Research and Training Center, FBI Academy, Quantico, VA 22135.

Abstract

A simple, practical approach for the extraction of PCR-amplifiable DNA for the HLA-DQ alpha gene from bloodstains deposited on various substrates is described. DNA from bloodstains is purified using Chelex 100 ion-exchange resin and then amplified. If amplification is not achieved, the extract is washed through a Centricon 100 dialysis/concentration tube. If the second amplification of this extract produces a negative result, the extract is processed with Chelex 100 again. This approach has been found to be reliable, safe, efficient and economical.

PMID:: 1911413; [PubMed - indexed for MEDLINE]

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Amplification of HLA-DQA1 gene from bloodstains by polymerase chain reaction. [Nihon Hoigaku Zasshi. 1993]
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