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1: J Clin Microbiol. 2009 Mar;47(3):674-9. Epub 2008 Dec 24.Click here to read Links

Simple, sensitive, and swift sequencing of complete H5N1 avian influenza virus genomes.

Höper D, Hoffmann B, Beer M.

Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Südufer 10, Greifswald-Insel Riems 17493, Germany.

The spread of highly pathogenic avian influenza A virus (HPAIV) of subtype H5N1 demands fast and reliable methods for in-depth, full-length sequence analysis. For this purpose, we designed a simple and sensitive method for the preparation of sequencing libraries from H5N1 HPAIV diagnostic RNA samples for sequencing with the Genome Sequencer FLX instrument. The method presented seamlessly integrates high-throughput pyrosequencing with the Roche/454 instrument into diagnostics without the need for additional equipment or molecular biological techniques besides standard PCR and the Genome Sequencer FLX sample preparation and sequencing pipeline.

PMID: 19109473 [PubMed - indexed for MEDLINE]

PMCID: PMC2650901 [Available on 2009/09/01]