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J Am Chem Soc. 2008 Dec 31;130(52):17664-5. doi: 10.1021/ja807430h.

A general and efficient method for the site-specific dual-labeling of proteins for single molecule fluorescence resonance energy transfer.

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  • 1Department of Molecular Biology, Department of Chemistry and the Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, California 92037, USA.

Abstract

A general strategy for the site-specific dual-labeling of proteins for single-molecule fluorescence resonance energy transfer is presented. A genetically encoded unnatural ketone amino acid was labeled with a hydroxylamine-containing fluorophore with high yield (>95%) and specificity. This methodology was used to construct dual-labeled T4 lysozyme variants, allowing the study of T4 lysozyme folding at single-molecule resolution. The presented strategy is anticipated to expand the scope of single-molecule protein structure and function studies.

PMID:
19108697
[PubMed - indexed for MEDLINE]
PMCID:
PMC2743202
Free PMC Article
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