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Am J Respir Cell Mol Biol. 2009 Aug;41(2):170-8. doi: 10.1165/rcmb.2008-0138OC. Epub 2008 Dec 18.

The antioxidant mimetic, MnTE-2-PyP, reduces intracellular growth of Mycobacterium abscessus.

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  • 1Department of Medicine, National Jewish Health, Denver, CO 80206, USA. oberleyr@njc.org

Abstract

Mycobacterium abscessus is a rapidly growing environmental mycobacterium that can cause severe skin, soft tissue, and lung infections. M. abscessus grows inside macrophages, and these cells release a vast number of proinflammatory cytokines in response to infections. The metalloporphyrin, MnTE-2-PyP, is a broad antioxidant that reduces inflammatory cell signaling. Macrophage-like THP-1 cells were infected with M. abscessus in the presence or absence of MnTE-2-PyP. MnTE-2-PyP significantly decreased, in a dose-dependent manner, the number of M. abscessus organisms recovered from infected THP-1 cells 4 and 8 days after infection. Furthermore, when combined with clarithromycin, MnTE-2-PyP additively reduced the number of cells associated with M. abscessus. A mechanism of bacterial growth inhibition by MnTE-2-PyP was then elucidated. It was found that MnTE-2-PyP promoted the survival of infected THP-1 cells and increased fusion of M. abscessus-containing phagosomes with lysosomes.

PMID:
19097985
[PubMed - indexed for MEDLINE]
PMCID:
PMC2715906
Free PMC Article
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