Abstract
In this paper, we show that mutants in the gene stambhA (stmA), which encodes a putative phosphatidylinositol 4,5 bisphosphate-diacylglycerol lipase, exhibit a significant reduction in the amplitudes of odor-evoked responses recorded from the antennal surface of adult Drosophila. This lends support to previously published findings that olfactory transduction in Drosophila requires a phospholipid intermediate. Mutations in stmA also affect the olfactory behavior response of larvae. Moreover, there is a requirement for G(q)alpha and phospholipase Cbeta function in larval olfaction. The results suggest that larval olfactory transduction, like that of the adult, utilizes a phospholipid second messenger, generated by the activation of G(q)alpha and Plcbeta21c, and modulated by the stmA gene product.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Animals, Genetically Modified
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Behavior, Animal
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Drosophila
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Drosophila Proteins / genetics*
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Drosophila Proteins / metabolism
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Electrophysiology
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GTP-Binding Protein alpha Subunits, Gq-G11 / genetics
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GTP-Binding Protein alpha Subunits, Gq-G11 / metabolism
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Ganglia, Invertebrate / cytology
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Ganglia, Invertebrate / growth & development
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Gene Expression Regulation, Developmental / genetics
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Green Fluorescent Proteins / genetics
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Larva / genetics
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Larva / physiology
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Lipoprotein Lipase / classification
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Lipoprotein Lipase / genetics*
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Mushroom Bodies / cytology
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Mushroom Bodies / growth & development
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Mutation / genetics*
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Neurons / metabolism
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Odorants
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Olfactory Pathways / growth & development*
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Olfactory Pathways / metabolism
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RNA Interference / physiology
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Signal Transduction / genetics*
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Smell / genetics
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Smell / physiology*
Substances
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Drosophila Proteins
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enhanced green fluorescent protein
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Green Fluorescent Proteins
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Lipoprotein Lipase
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GTP-Binding Protein alpha Subunits, Gq-G11