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Methods Mol Biol. 2009;470:71-83. doi: 10.1007/978-1-59745-204-5_7.

Isolation and purification of antigenic components of Cryptococcus.

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  • 1Department of Medicine and Microbiology, University of Massachusetts Medical School, Worcester, Massachusetts, USA.

Abstract

The encapsulated fungal pathogens Cryptococcus neoformans and Cryptococcus gattii are significant agents of life-threatening infections, particularly in persons with suppressed cell-mediated immunity. This chapter provides detailed methodology for the purification of two of the major antigen fractions of C. neoformans: glucuronoxylomannan (GXM) and mannoprotein (MP). GXM is the primary component of the polysaccharide capsule, which is the major cryptococcal virulence factor. In contrast, MPs have been identified as key antigens that stimulate T-cell responses. Purification of GXM and MP should assist investigators studying the antigenic, biochemical, and virulence properties of Cryptococcus species.

PMID:
19089377
[PubMed - indexed for MEDLINE]
PMCID:
PMC3297022
Free PMC Article

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