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Forensic Sci Int Genet. 2008 Mar;2(2):126-33. doi: 10.1016/j.fsigen.2007.10.010. Epub 2007 Nov 9.

2006 GEP-ISFG collaborative exercise on mtDNA: reflections about interpretation, artefacts, and DNA mixtures.

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  • 1Comisaría General de Policía Científica, DNA Laboratory, Madrid, Spain. lourditasmt@ya.com

Abstract

We report the results of the seventh edition of the GEP-ISFG mitochondrial DNA (mtDNA) collaborative exercise. The samples submitted to the participant laboratories were blood stains from a maternity case and simulated forensic samples, including a case of mixture. The success rate for the blood stains was moderate ( approximately 77%); even though four inexperienced laboratories concentrated about one-third of the total errors. A similar success was obtained for the analysis of mixed samples (78.8% for a hair-saliva mixture and 69.2% for a saliva-saliva mixture). Two laboratories also dissected the haplotypes contributing to the saliva-saliva mixture. Most of the errors were due to reading problems and misinterpretation of electropherograms, demonstrating once more that the lack of a solid devised experimental approach is the main cause of error in mtDNA testing.

PMID:
19083807
[PubMed - indexed for MEDLINE]
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