We conducted an on-line survey of 164 local health departments' staff in five Northwestern states in 2006-2007 to assess Internet access and use by staff. Most (96%) respondents had full-time access to their own worksite computer. The most important selection criterion for selecting Web sites was credibility of the sponsoring organization (55%). Accuracy (46%), reputable source (30%), and currency of information (19%) were considered most critical for assessing information quality. Centers for Disease Control and Prevention (80%) and state health department (60%) sites were used most commonly. These findings can be used to improve public health Web sites and support decision making in practice.