Determination of the branch location of extra N-acetyllactosamine units in sialo N-linked tetraantennary oligosaccharides

FEBS Lett. 1991 Jul 29;286(1-2):18-24. doi: 10.1016/0014-5793(91)80931-r.

Abstract

An approach is presented for the determination of the branch location of 1 or 2 extra N-acetyllactosamine units in sialo N-linked carbohydrate chains from glycoproteins. Tetraantennary oligosaccharides containing extra N-acetyllactosamine units were digested with endo-beta-galactosidase, followed by treatment with N-acetyl-beta-glucosaminidase, yielding products which could be analysed by 1H-NMR spectroscopy, thereby giving conclusive data about the location of the extra units in the intact structures.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylglucosaminidase / metabolism
  • Amino Sugars / chemistry*
  • Amino Sugars / metabolism
  • Animals
  • Carbohydrate Sequence
  • Cell Line
  • Cricetinae
  • Erythropoietin / chemistry
  • Glycoside Hydrolases*
  • Humans
  • Magnetic Resonance Spectroscopy
  • Molecular Sequence Data
  • Oligosaccharides / chemistry*
  • Oligosaccharides / metabolism
  • beta-Galactosidase / metabolism

Substances

  • Amino Sugars
  • Oligosaccharides
  • sialooligosaccharides
  • Erythropoietin
  • N-acetyllactosamine
  • Glycoside Hydrolases
  • keratan-sulfate endo-1,4-beta-galactosidase
  • beta-Galactosidase
  • Acetylglucosaminidase