Methods Mol Biol. 2009;501:69-76.

Enumeration of bacteriophages by double agar overlay plaque assay.

Kropinski AM, Mazzocco A, Waddell TE, Lingohr E, Johnson RP.

Source

Public Health Agency of Canada, Laboratory for Foodborne Diseases, Guelph, Ontario, Canada.

Abstract

The determination of the concentration of infectious phage particles is fundamental to many protocols in phage biology, genetics, and molecular biology. In this chapter the classical overlay protocol is described.

PMID:: 19066811; [PubMed - indexed for MEDLINE]

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Enumeration of bacteriophages by double agar overlay plaque assay.
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Enumeration of bacteriophages by double agar overlay plaque assay.

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