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    J Vis Exp. 2008 Aug 23;(18). pii: 889. doi: 10.3791/889.

    Protocols for microapplicator-assisted infection of lepidopteran larvae with baculovirus.

    Source

    Department of Entomology, Iowa State University, USA. hrli@iastate.edu

    Abstract

    Baculoviruses are widely used both as protein expression vectors and as insect pest control agents. . This video shows how lepidopteran larvae can be infected with microapplicator techniques in the gut with baculovirus polyhedra and in the hemolymph with budded virus. This accompanying Springer Protocols section provides an overview of the baculovirus lifecycle and use of baculoviruses as insecticidal agents. Formulation and application of baculoviruses for pest control purposes are described elsewhere.

    PMID:
    19066502
    [PubMed - indexed for MEDLINE]
    PMCID:
    PMC3253561
    Free PMC Article

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