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Biochem Biophys Res Commun. 2009 Jan 16;378(3):539-44. Epub 2008 Dec 3.

Flow cytometric and immunohistochemical detection of in vivo BrdU-labeled cells in mouse fat depots.

Staszkiewicz J, Gimble J, Cain C, Dietrich M, Burk D, Kirk-Ballard H, Gawronska-Kozak B.

Source

Pennington Biomedical Research Center, Louisiana State University System, 6400 Perkins Rd., Baton Rouge, LA 70808, USA.

Abstract

This study has determined the natural frequency and localization of progenitor/stem cells within fat depots in situ based on their ability to retain DNA nucleotide label (BrdU). Neonate and mature male C57BL6/J mice were injected intraperitoneally with BrdU- and label-retaining cells (LRC) were quantified in fat depots by immunohistochemical, immunofluorescent, and flow cytometric methods. In neonates, LRC constituted 27% of the cells in inguinal fat (iWAT) and 65% in interscapular brown fat (BAT) after Day 10 and 26% of the cells in epididymal fat (eWAT) after Day 28. After 52 days, the LRC accounted for 0.72% of iWAT, 0.53% of eWAT and 1.05% of BAT, respectively. The BrdU-labeled cells localized to two areas: single cells distributed among adipocytes or those adjacent to the blood vessels wall. In mature C57BL6/J mice, flow cytometric analysis determined that a majority of the LRC were also positive for stem cell antigen-1 (Sca-1).

PMID:: 19056354; [PubMed - indexed for MEDLINE]
PMCID: PMC2731488

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