S100B secretion is stimulated by IL-1beta in glial cultures and hippocampal slices of rats: Likely involvement of MAPK pathway

J Neuroimmunol. 2009 Jan 3;206(1-2):52-7. doi: 10.1016/j.jneuroim.2008.10.012. Epub 2008 Nov 29.

Abstract

S100B is an astrocyte-derived cytokine implicated in the IL-1beta-triggered cytokine cycle in Alzheimer's disease. However, the secretion of S100B following stimulation by IL-1beta has not been directly demonstrated. We investigated S100B secretion in cortical primary astrocyte cultures, C6 glioma cells and acute hippocampal slices exposed to IL-1beta. S100B secretion was induced by IL-1beta in all preparations, involving MAPK pathway and, apparently, NF-small ka, CyrillicB signaling. Astrocytes and C6 cells exhibited different sensitivities to IL-1beta. These results suggest that IL-1beta-induced S100B secretion is a component of the neuroinflammatory response, which would support the involvement of S100B in the genesis of neurodegenerative diseases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Animals
  • Animals, Newborn
  • Cells, Cultured
  • Cerebral Cortex / cytology
  • Dose-Response Relationship, Drug
  • Enzyme Inhibitors / pharmacology
  • Hippocampus / drug effects*
  • In Vitro Techniques
  • Indoles
  • Interleukin-1beta / pharmacology*
  • L-Lactate Dehydrogenase / metabolism
  • Mitogen-Activated Protein Kinase Kinases / metabolism*
  • Nerve Growth Factors / metabolism*
  • Neuroglia / drug effects*
  • Nitric Oxide / metabolism
  • Rats
  • Rats, Wistar
  • S100 Calcium Binding Protein beta Subunit
  • S100 Proteins / metabolism*
  • Signal Transduction / drug effects
  • Signal Transduction / physiology*
  • Subcellular Fractions / drug effects
  • Time Factors

Substances

  • Enzyme Inhibitors
  • Indoles
  • Interleukin-1beta
  • Nerve Growth Factors
  • S100 Calcium Binding Protein beta Subunit
  • S100 Proteins
  • S100b protein, rat
  • Nitric Oxide
  • DAPI
  • L-Lactate Dehydrogenase
  • Mitogen-Activated Protein Kinase Kinases