Cell-Specific Venus Expression in Transgenic Mice
(A) Colocalization of Venus fluorescence (green) with glucagon immunofluorescence (red) in the small intestine. Scale bar, 20 μm.
(B) L cells were collected by FACS sorting with gates on pulse width, side and forward scatter to select single cells and yellow (580 nm) and green (530 nm) fluorescence to select Venus-positive (R1) or Venus-negative (R2) cells (excitation 488 nm). The figure shows a representative sort from the small intestine.
(C) Relative expression of proglucagon and Pyy mRNAs in GLUTag cells and L_pos and L_neg cells from different regions of the intestine (middle third of small intestine [mid S1], lower third of small intestine [lower S1], and colon). Data are presented as geometric mean and upper SE (n ≥ 3 each). ^∗∗p < 0.01, ^∗∗∗p < 0.001 by Student's t test.
(D) GIP, GLP-1, and PYY peptide contents in L_pos and L_neg cells from the small intestine (upper half, Up SI) or colon, as indicated, collected by FACS sorting and measured by ELISA. Data represent mean and SE of ≥ 3 samples with statistical comparisons between corresponding L_pos and L_neg cells. ^∗∗p < 0.01, ^∗∗∗p < 0.001. ND, not detected.

GLP-1 Secretion from Primary Intestinal Cultures
(A and B) Mixed primary cultures from the upper half of the small intestine (A) or the colon (B) were incubated in bath solution containing the glucose concentrations indicated. The percentage of GLP-1 secretion in each well is expressed relative to the basal secretion measured in parallel on the same day. Dose-response curves were fitted through the entire data set using a logistic equation, giving EC₅₀s of 4 mM and 0.7 mM for the small intestine and colon, respectively (not significantly different). Error bars represent 1 SE, and significance is shown relative to baseline using a single-factor t test. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001.
(C) GLP-1 secretion from primary colonic cultures triggered by forskolin/IBMX (F/I, 10 μM of each), bombesin (Bb, 100 nM), or glucose (Gluc, 10 mM), as indicated, measured and expressed as in (A). Error bars represent 1 SE, and significance is shown relative to baseline using a single-factor t test: ^∗∗∗p < 0.001. The additional effect of glucose was analyzed by regression analysis to compensate for variation in the effectiveness of forskolin/IBMX or bombesin between different experiments: ΔΔΔp < 0.001.
(D and E) GLP-1 secretion from the upper small intestine (D) or colon (E), measured as in (A), following addition of glucose (Gluc, 10 mM), αMG (10 mM), tolbutamide (tolb, 500 μM), sucralose (sucl, 1 or 20 mM), or acesulfame K (AceK, 2 mM). The control bar (Con) and dashed line indicate the basal rate of secretion. Data represent the mean and SE of the number of wells indicated, and significance is shown relative to baseline, tested by a single-factor t test. ^∗p < 0.05, ^∗∗ p < 0.01. n.s., not significant.

Electrical Activity and [Ca²⁺]_i in Cultured Colonic L Cells
(A) Colonic epithelial cells fixed after 10 days in primary culture. DIC, DAPI (blue), Venus (green).
(B) Glucose-triggered electrical activity in L cells. (Left) Representative whole-cell perforated patch current clamp recording of a Venus-positive cell stimulated with glucose (10 mM) applied as indicated. (Right) Average action potential frequency before (C1), during (Gluc), and after (C2) application of glucose measured in eight cells. Error bars represent 1 SE and significance, between AP-frequency in the presence and absence of glucose was tested by Student's paired t test. ^∗∗p < 0.01.
(C) Average action potential frequency before (C1), during (tolb), and after (C2) application of tolbutamide (500 μM) measured in nine cells, as in (B). Error bars represent 1 SE, and significance between AP-frequency in the presence and absence of tolbutamide was tested by Student's paired t test. ^∗∗p < 0.01.
(D) Functional K_ATP wash-out currents in cultured L cells. (Left) Slope conductances between −70 and −50 mV were recorded from a cell in conventional whole-cell voltage clamp. The arrow indicates the time when the cell attached mode was converted into conventional whole cell with 300 μM ATP in the pipette. Tolbutamide (tolb, 500 μM) was applied at steady state, as indicated. (Right) Average conductances measured as exemplified on the left in five cells at times (a) cell attached, (b) wash-out steady state, and (c) after application of tolbutamide. Error bars represent 1 SE, and significance was tested by Student's paired t test. ^∗∗∗p < 0.001.
(E) L_pos and L_neg cells in colonic cultures were loaded with fura2-AM and identified by their presence/absence of Venus fluorescence (475 nm excitation, left). The image of fura2-loaded cells excited at 340 nm (right) was used to outline L_pos (green) and L_neg (red) cells.
(F) The 340/380 nm fluorescence ratios (reflecting [Ca²⁺]_i) of the two cells outlined in (E) are shown following addition of 10 mM glucose to the perfusate. Green trace, L_pos cell; red trace, L_neg cell.
(G) Mean calcium changes in L_pos (green bars) and L_neg (red bars) cells, identified and monitored as in (E) and (F), following the addition of glucose (Gluc, 10 mM), αMG (10 mM), tolbutamide (tolb, 100 μM), KCl (30 mM), sucralose (sucl, 1 or 20 mM), bombesin (Bb, 100 nM), and forskolin/IBMX (F/I, 10 μM of each), as indicated. 340/380 ratios in the presence of the test agent were normalized to the mean of the background ratios of each cell measured before addition and after washout of the test compound. Data represent the mean and SE of the number of cells indicated above each bar. Δp < 0.05, ΔΔp < 0.01, and ΔΔΔp < 0.001 compared with baseline. ^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001 for comparison between corresponding L_pos and L_neg cells by Student's t test.

Expression Analysis
Expression of the genes indicated in GLUTag cells, L_pos cells, and L_neg cells from three different regions of the gut (middle third of small intestine [SI], lower third of small intestine, and colon) and in pancreatic α, β, and δ/PP cells isolated as in Figure S2. Expression was normalized to that of β-actin in the same sample. Data are presented as geometric mean and upper SE (n ≥ 3 each). L_pos and L_neg cells were compared by Student's t tests: ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001. n.s., not significant.