The S. cerevisiae proteome was grouped into three categories, the highly structured (S), moderately unstructured (M) and the highly unstructured (U) category based on the fraction of unstructured residues over the entire protein length.

Box-plots for various regulatory and cellular properties for the three different groups of proteins (S, M and U) in S. cerevisiae. Each box-plot identifies the middle 50% of the data, the median, and the extreme points. The entire set of datapoints is divided into quartiles and the interquartile range (IQR) is calculated as the difference between x_0.75 and x_0.25. The range of the 25% of the data points above (x_0.75) and below (x_0.25) the median (x_0.50) is displayed as a filled box. The horizontal line and the notch represent the median and confidence intervals, respectively. Datapoints greater or less than 1.5*IQR represent outliers and are shown as dots. The horizontal line that is connected by dashed lines above and below the filled box (whiskers) represents the largest and the smallest non-outlier datapoints, respectively. (A) transcriptional complexity, (B) mRNA abundance, (C) mRNA half-life, (D) poly-A tail length (percentage of transcripts that have short poly-A tail length within a group), (E) ribosomal density, (F) protein abundance, (G) protein half-life, (H) PEST-sequence content (percentage of proteins with PEST motif within a group), (I) TATA box content (percentage of genes with a TATA box within a group) and (J) noise (distance from median co-efficient of variation) in protein production. ⁺ denotes statistically significant differences between the S and U group (table S3).

Box-plots for various regulatory and cellular parameters for the three groups of proteins (S, M and U) in S. pombe (A-D) and humans (E-G). All reported differences are statistically significant (table S4).