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    Vet Microbiol. 2009 Apr 14;136(1-2):8-19. Epub 2008 Nov 1.

    Characterization of an equine macrophage cell line: application to studies of EIAV infection.

    Fidalgo-Carvalho I, Craigo JK, Barnes S, Costa-Ramos C, Montelaro RC.

    Source

    Iron Genes and the Immune System, Instituto de Biologia Molecular e Celular, Rua do Campo Alegre, Oporto, Portugal.

    Abstract

    EIAV is a monocyte/macrophage tropic virus. To date, even though EIAV has been under investigation for numerous years, very few details have been elucidated about EIAV/macrophage interactions. This is largely due to the absence of an equine macrophage cell line that would support viral replication. Herein we describe the spontaneous immortalization and generation of a clonal equine macrophage-like (EML) cell line with the functional and immunophenotype characteristics of differentiated equine monocyte derived macrophage(s) (eMDM(s)). These cells possess strong non-specific esterase (NSE) activity, are able to phagocytose fluorescent bioparticles, and produce nitrites in response to LPS. The EML-3C cell line expresses the EIAV receptor for cellular entry (ELR1) and supports replication of the virulent EIAV(PV) biological clone. Thus, EML-3C cells provide a useful cell line possessing equine macrophage related properties for the growth and study of EIAV infection as well as of other equine macrophage tropic viruses.

    PMID:
    19038510
    [PubMed - indexed for MEDLINE]
    PMCID: PMC2689946
    Free PMC Article

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