Your browser version may not work well with NCBI's Web applications. More information here...
1: J Chromatogr A. 2008 Dec 26;1215(1-2):107-15. Epub 2008 Nov 7.Links

Analysis of nine drugs and their cytochrome P450-specific probe metabolites from urine by liquid chromatography-tandem mass spectrometry utilizing sub 2 microm particle size column.

Petsalo A, Turpeinen M, Pelkonen O, Tolonen A.

Novamass Ltd, Medipolis Center, Kiviharjuntie 11, 90220 Oulu, Finland.

An LC/MS/MS method was developed for the analysis of twelve cytochrome P450 (CYP)-specific probe metabolites and their nine parent drugs from human urine. CYP-specific metabolites of melatonin (CYP1A2), nicotine (CYP2A6), bupropion (CYP2B6), repaglinide (CYP2C8), losartan (CYP2C9), omeprazole (CYP2C19 and CYP3A4), dextromethorphan (CYP2D6), chlorzoxazone (CYP2E1) and midazolam (CYP3A4) were all analyzed using the same LC/MS/MS method with a single analytical run, either after a one-at-a-time dose or cocktail-type dosing of the parent drugs. Ultra performance liquid chromatography (UPLC) with a 1.7 microm particle size column was utilized, providing 1.5-3-fold increase in sensitivity, decrease of analysis time to one third and clearly better chromatographic peak shapes when comparing it with the method using traditional high performance liquid chromatography for the same metabolites. In addition, the method was applied for the analysis of the metabolites from human urine samples collected at multiple time points after single and N-in-one dosing of each of the drugs, showing that the use of both the analytical method and these probe metabolites as CYP-specific markers is feasible in in vivo drug-drug interaction or phenotyping studies.

PMID: 19019380 [PubMed - indexed for MEDLINE]

Patient Drug Information

  • Esomeprazole (Nexium® )

    Esomeprazole is used to treat gastroesophageal reflux disease (GERD), a condition in which backward flow of acid from the stomach causes heartburn and injury of the esophagus (food pipe between the mouth and stomach) in ...

  • Omeprazole (Prilosec® , Prilosec® OTC, Zegerid® )

    Prescription omeprazole is used alone or with other medications to treat ulcers (sores in the lining of the stomach or small intestine); gastroesophageal reflux disease (GERD), a condition in which backward flow of acid ...

  • Midazolam (available generically)

    Midazolam is given to children before medical procedures or before anesthesia for surgery to cause drowsiness, relieve anxiety, and prevent any memory of the event. Midazolam is in a class of medications called benzodiaz...

  • » See all 11 drug reports ...
  • Source: AHFS Consumer Medication Information