Proc Natl Acad Sci U S A. 2008 Nov 25;105(47):18255-60. Epub 2008 Nov 18.

A functional proteomics approach links the ubiquitin-related modifier Urm1 to a tRNA modification pathway.

Schlieker CD, Van der Veen AG, Damon JR, Spooner E, Ploegh HL.

Source

Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA. schlieke@wi.mit.edu

Abstract

Urm1 is a highly conserved ubiquitin-related modifier of unknown function. A reduction of cellular Urm1 levels causes severe cytokinesis defects in HeLa cells, resulting in the accumulation of enlarged multinucleated cells. To understand the underlying mechanism, we applied a functional proteomics approach and discovered an enzymatic activity that links Urm1 to a tRNA modification pathway. Unlike ubiquitin (Ub) and many Ub-like modifiers, which are commonly conjugated to proteinaceous targets, Urm1 is activated by an unusual mechanism to yield a thiocarboxylate intermediate that serves as sulfur donor in tRNA thiolation reactions. This mechanism is reminiscent of that used by prokaryotic sulfur carriers and thus defines the evolutionary link between ancient Ub progenitors and the eukaryotic Ub/Ub-like modification systems.

PMID:: 19017811; [PubMed - indexed for MEDLINE]
PMCID:: PMC2584574

Free PMC Article

Images from this publication.See all images (4) Free text

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

Supplemental Content

Save items

Related citations in PubMed

Role of the ubiquitin-like protein Urm1 as a noncanonical lysine-directed protein modifier. [Proc Natl Acad Sci U S A. 2011]
Role of the ubiquitin-like protein Urm1 as a noncanonical lysine-directed protein modifier.
Van der Veen AG, Schorpp K, Schlieker C, Buti L, Damon JR, Spooner E, Ploegh HL, Jentsch S. Proc Natl Acad Sci U S A. 2011 Feb 1; 108(5):1763-70. Epub 2011 Jan 5.
Review The dual role of ubiquitin-like protein Urm1 as a protein modifier and sulfur carrier. [Protein Cell. 2011]
Review The dual role of ubiquitin-like protein Urm1 as a protein modifier and sulfur carrier.
Wang F, Liu M, Qiu R, Ji C. Protein Cell. 2011 Aug; 2(8):612-9. Epub 2011 Sep 9.
Ubiquitin-related modifier Urm1 acts as a sulphur carrier in thiolation of eukaryotic transfer RNA. [Nature. 2009]
Ubiquitin-related modifier Urm1 acts as a sulphur carrier in thiolation of eukaryotic transfer RNA.
Leidel S, Pedrioli PG, Bucher T, Brost R, Costanzo M, Schmidt A, Aebersold R, Boone C, Hofmann K, Peter M. Nature. 2009 Mar 12; 458(7235):228-32. Epub 2009 Jan 14.
Review Urm1 at the crossroad of modifications. 'Protein Modifications: Beyond the Usual Suspects' Review Series. [EMBO Rep. 2008]
Review Urm1 at the crossroad of modifications. 'Protein Modifications: Beyond the Usual Suspects' Review Series.
Pedrioli PG, Leidel S, Hofmann K. EMBO Rep. 2008 Dec; 9(12):1196-202.
Review Putting proteomics on target: activity-based profiling of ubiquitin and ubiquitin-like processing enzymes. [Expert Rev Proteomics. 2006]
Review Putting proteomics on target: activity-based profiling of ubiquitin and ubiquitin-like processing enzymes.
Kessler BM. Expert Rev Proteomics. 2006 Apr; 3(2):213-21.

See reviews... See all...

Cited by 16 PubMed Central articles

Review Uncovering ubiquitin and ubiquitin-like signaling networks. [Chem Rev. 2011]
Review Uncovering ubiquitin and ubiquitin-like signaling networks.
Vertegaal AC. Chem Rev. 2011 Dec 14; 111(12):7923-40. Epub 2011 Oct 17.
E1- and ubiquitin-like proteins provide a direct link between protein conjugation and sulfur transfer in archaea. [Proc Natl Acad Sci U S A. 2011]
E1- and ubiquitin-like proteins provide a direct link between protein conjugation and sulfur transfer in archaea.
Miranda HV, Nembhard N, Su D, Hepowit N, Krause DJ, Pritz JR, Phillips C, Söll D, Maupin-Furlow JA. Proc Natl Acad Sci U S A. 2011 Mar 15; 108(11):4417-22. Epub 2011 Feb 28.
Urm1 couples sulfur transfer to ubiquitin-like protein function in oxidative stress. [Proc Natl Acad Sci U S A. 2011]
Urm1 couples sulfur transfer to ubiquitin-like protein function in oxidative stress.
Petroski MD, Salvesen GS, Wolf DA. Proc Natl Acad Sci U S A. 2011 Feb 1; 108(5):1749-50. Epub 2011 Jan 18.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
BioSystems
Pathways and biological systems (BioSystems) that cite the current articles. Citations are from the BioSystems source databases (KEGG and BioCyc).
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
Gene (GeneRIF)
Gene records that have the current articles as Reference into Function citations (GeneRIFs). NLM staff reviewing the literature while indexing MEDLINE add GeneRIFs manually.
Gene (OMIM)
Gene records associated with Online Mendelian Inheritance in Man (OMIM) records that cite the current articles in their reference lists.
HomoloGene
HomoloGene clusters of homologous genes and sequences that cite the current articles. These are references on the Gene and sequence records in the HomoloGene entry.
Nucleotide
Primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
Nucleotide (RefSeq)
NCBI nucleotide Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
OMIM (calculated)
Online Mendelian Inheritance in Man (OMIM) records that include the current articles as references in the light bulb links within or in the citations at the end of the OMIM record. The references available through the light bulb link are collected using the PubMed related articles algorithm to identify records with similar terminology to the OMIM record.
OMIM (cited)
Online Mendelian Inheritance in Man (OMIM) records that include the current articles as reference cited at the end of the OMIM record.
Protein (RefSeq)
NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
References for this PMC Article
Citation referenced in PubMed article. Only valid for PubMed citations that are also in PMC.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
UniGene
UniGene clusters of expressed sequences that are associated with the current articles through references on the clustered sequence records and related Gene records.
Protein
Protein translation features of primary database (GenBank) nucleotide records reported in the current articles as well as Reference Sequences (RefSeqs) that include the articles as references.
GEO Profiles
Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile.
Free in PMC
Free full text articles in PMC
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

A functional proteomics approach links the ubiquitin-related modifier Urm1 to a ...
A functional proteomics approach links the ubiquitin-related modifier Urm1 to a tRNA modification pathway.
Proc Natl Acad Sci U S A. 2008 Nov 25 ;105(47):18255-60. Epub 2008 Nov 18 .

PubMed
Evidence for a human-specific mechanism for diet and antibody-mediated inflammat...
Evidence for a human-specific mechanism for diet and antibody-mediated inflammation in carcinoma progression.
Proc Natl Acad Sci U S A. 2008 Dec 2 ;105(48):18936-41. Epub 2008 Nov 18 .

PubMed
Metagenomic analysis indicates that stressors induce production of herpes-like v...
Metagenomic analysis indicates that stressors induce production of herpes-like viruses in the coral Porites compressa.
Proc Natl Acad Sci U S A. 2008 Nov 25 ;105(47):18413-8. Epub 2008 Nov 18 .

PubMed
Full accounting of diabetes and pre-diabetes in the U.S. population in 1988-1994...
Full accounting of diabetes and pre-diabetes in the U.S. population in 1988-1994 and 2005-2006.
Diabetes Care. 2009 Feb ;32(2):287-94. Epub 2008 Nov 18 .

PubMed
Use of the tyrosine kinase inhibitor sunitinib in a patient with von Hippel-Lind...
Use of the tyrosine kinase inhibitor sunitinib in a patient with von Hippel-Lindau disease: targeting angiogenic factors in pheochromocytoma and other von Hippel-Lindau disease-related tumors.
J Clin Endocrinol Metab. 2009 Feb ;94(2):386-91. Epub 2008 Nov 18 .

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: