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J Microsc. 2008 Oct;232(1):1-6. doi: 10.1111/j.1365-2818.2008.02110.x.

Mirrored pyramidal wells for simultaneous multiple vantage point microscopy.

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  • 1Department of Biological Sciences, Vanderbilt University, VU Station B 351807, Nashville, TN 37235-1807, USA.

Abstract

We report a novel method for obtaining simultaneous images from multiple vantage points of a microscopic specimen using size-matched microscopic mirrors created from anisotropically etched silicon. The resulting pyramidal wells enable bright-field and fluorescent side-view images, and when combined with z-sectioning, provide additional information for 3D reconstructions of the specimen. We have demonstrated the 3D localization and tracking over time of the centrosome of a live Dictyostelium discoideum. The simultaneous acquisition of images from multiple perspectives also provides a five-fold increase in the theoretical collection efficiency of emitted photons, a property which may be useful for low-light imaging modalities such as bioluminescence, or low abundance surface-marker labelling.

PMID:
19017196
[PubMed - indexed for MEDLINE]
PMCID:
PMC3789065
Free PMC Article

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