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Curr Protoc Microbiol. 2008 Nov;Chapter 3:Unit 3A.5. doi: 10.1002/9780471729259.mc03a05s11.

Laboratory maintenance of Rickettsia rickettsii.

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  • 1Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland, USA.


This unit includes protocols for the laboratory maintenance of the obligate intracellular bacterium Rickettsia rickettsii, including propagation in mammalian cell cultures, as well as isolation, counting, and storage procedures. Regulations for working with R. rickettsii in biosafety level 3 containment are also discussed.

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