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1: Appl Environ Microbiol. 2009 Jan;75(2):534-7. Epub 2008 Nov 14.Click here to read Click here to read Links

Characterization of denitrification gene clusters of soil bacteria via a metagenomic approach.

Demanèche S, Philippot L, David MM, Navarro E, Vogel TM, Simonet P.

Environmental Microbial Genomics Group, Laboratoire AMPERE, UMR CNRS 5005, Ecole Centrale de Lyon, Université de Lyon, 36 Avenue Guy de Collongue, 69134 Ecully, France.

We characterized operons encoding enzymes involved in denitrification, a nitrogen-cycling process involved in nitrogen losses and greenhouse gas emission, using a metagenomic approach which combines molecular screening and pyrosequencing. Screening of 77,000 clones from a soil metagenomic library led to the identification and the subsequent characterization of nine denitrification gene clusters.

PMID: 19011059 [PubMed - indexed for MEDLINE]

PMCID: PMC2620714