Display Settings:

Format

Send to:

Choose Destination
See comment in PubMed Commons below
Mol Biol Cell. 2009 Jan;20(1):379-88. doi: 10.1091/mbc.E07-10-1007. Epub 2008 Nov 12.

Katanin knockdown supports a role for microtubule severing in release of basal bodies before mitosis in Chlamydomonas.

Author information

  • 1Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, BC, Canada.

Abstract

Katanin is a microtubule-severing protein that participates in the regulation of cell cycle progression and in ciliary disassembly, but its precise role is not known for either activity. Our data suggest that in Chlamydomonas, katanin severs doublet microtubules at the proximal end of the flagellar transition zone, allowing disengagement of the basal body from the flagellum before mitosis. Using an RNA interference approach we have discovered that severe knockdown of the p60 subunit of katanin, KAT1, is achieved only in cells that also carry secondary mutations that disrupt ciliogenesis. Importantly, we observed that cells in the process of cell cycle-induced flagellar resorption sever the flagella from the basal bodies before resorption is complete, and we find that this process is defective in KAT1 knockdown cells.

PMID:
19005222
[PubMed - indexed for MEDLINE]
PMCID:
PMC2613114
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for HighWire Icon for PubMed Central
    Loading ...
    Write to the Help Desk