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Cytotechnology. 2008 Mar;56(3):145-9. doi: 10.1007/s10616-008-9143-2. Epub 2008 Jun 24.

A method for measuring mitochondrial mass and activity.

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  • 1Dipartimento di Biologia Cellulare e dello Sviluppo "A. Monroy", Università degli Studi di Palermo, Viale delle Scienze ed. 16, 90128, Palermo, Italy.

Abstract

Mitochondria, responsible for the energy-generating process essential for the cell metabolism, differ for the number, localization and activity in animal cells and tissues in relation to the energetic needs. Using fluorescent probes specific for mitochondria, Mitotracker Green (MTG) and Orange (MTO), and Confocal Laser-Scanning Microscope (CLSM), we elaborated a method to measure in vivo the mitochondrial mass and activity, in sea urchin Paracentrotus lividus eggs and embryos. The analysis of captured images, revealed a variation of mitochondrial distribution and an increase of activity after fertilization.

PMID:
19002852
[PubMed]
PMCID:
PMC2553627
Free PMC Article

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