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Dev Cell. 2008 Nov;15(5):781-91. doi: 10.1016/j.devcel.2008.09.007.

Atlas of gene expression in the developing kidney at microanatomic resolution.

Author information

  • 1Division of Developmental Biology, Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH 45229, USA.

Erratum in

  • Dev Cell. 2009 Mar;16(3):482. Yu, Jing [added].

Abstract

Kidney development is based on differential cell-type-specific expression of a vast number of genes. While multiple critical genes and pathways have been elucidated, a genome-wide analysis of gene expression within individual cellular and anatomic structures is lacking. Accomplishing this could provide significant new insights into fundamental developmental mechanisms such as mesenchymal-epithelial transition, inductive signaling, branching morphogenesis, and segmentation. We describe here a comprehensive gene expression atlas of the developing mouse kidney based on the isolation of each major compartment by either laser capture microdissection or fluorescence-activated cell sorting, followed by microarray profiling. The resulting data agree with known expression patterns and additional in situ hybridizations. This kidney atlas allows a comprehensive analysis of the progression of gene expression states during nephrogenesis, as well as discovery of potential growth factor-receptor interactions. In addition, the results provide deeper insight into the genetic regulatory mechanisms of kidney development.

PMID:
19000842
[PubMed - indexed for MEDLINE]
PMCID:
PMC2653061
Free PMC Article
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