Cloning of the gene encoding alpha-methylserine hydroxymethyltransferase from Aminobacter sp. AJ110403 and Ensifer sp. AJ110404 and characterization of the recombinant enzyme

Hiroyuki Nozaki; Shinji Kuroda; Kunihiko Watanabe; Kenzo Yokozeki

doi:10.1271/bbb.80255

Cloning of the gene encoding alpha-methylserine hydroxymethyltransferase from Aminobacter sp. AJ110403 and Ensifer sp. AJ110404 and characterization of the recombinant enzyme

Biosci Biotechnol Biochem. 2008 Nov;72(11):3002-5. doi: 10.1271/bbb.80255. Epub 2008 Nov 7.

Authors

Hiroyuki Nozaki¹, Shinji Kuroda, Kunihiko Watanabe, Kenzo Yokozeki

Affiliation

¹ AminoScience Laboratories, Ajinomoto Co., Inc, Kawasaki, Japan.

PMID: 18997407
DOI: 10.1271/bbb.80255

Abstract

Genes encoding alpha-methylserine hydroxymethyltransferase from Aminobacter sp. AJ110403 and Ensifer sp. AJ110404 were cloned and expressed in Escherichia coli. The purified enzymes were homodimers with a 46-kDa subunit and contained 1 mol/mol-subunit of pyridoxal 5'-phosphate. The V(max) of these enzymes catalyzing the conversion of alpha-methyl-L-serine to D-alanine via tetrahydrofolate was 22.1 U/mg (AJ110403) and 15.4 U/mg (AJ110404).

MeSH terms

Alphaproteobacteria / enzymology*
Alphaproteobacteria / genetics*
Amino Acid Sequence
Chemical Precipitation
Cloning, Molecular
Glycine Hydroxymethyltransferase / chemistry
Glycine Hydroxymethyltransferase / genetics*
Glycine Hydroxymethyltransferase / isolation & purification
Glycine Hydroxymethyltransferase / metabolism
Molecular Sequence Data
Recombinant Proteins / chemistry
Recombinant Proteins / genetics*
Recombinant Proteins / isolation & purification
Recombinant Proteins / metabolism
Sinorhizobium / enzymology*
Sinorhizobium / genetics*

Substances

Recombinant Proteins
Glycine Hydroxymethyltransferase