Abstract
As part of an effort to elucidate the molecular basis for the pathogenesis of NKX2.5 mutations in congenital heart disease using X-ray crystallography, the NKX2.5 homeodomain has been crystallized in complex with a specific DNA element, the -242 promoter region of atrial natriuretic factor. Crystals of the homeodomain-DNA complex diffracted X-rays to 1.7 A resolution and belonged to space group P6(5), with unit-cell parameters a = b = 71.5, c = 94.3 A. The asymmetric unit contained two molecules of the NKX2.5 homeodomain and one double-stranded oligonucleotide.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Atrial Natriuretic Factor / genetics
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Crystallization
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DNA / chemistry*
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Homeobox Protein Nkx-2.5
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Homeodomain Proteins / chemistry*
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Homeodomain Proteins / genetics
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Humans
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Macromolecular Substances / chemistry
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Molecular Sequence Data
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Nucleic Acid Conformation
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Protein Conformation
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / genetics
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Transcription Factors / chemistry*
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Transcription Factors / genetics
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X-Ray Diffraction
Substances
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Homeobox Protein Nkx-2.5
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Homeodomain Proteins
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Macromolecular Substances
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NKX2-5 protein, human
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Recombinant Fusion Proteins
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Transcription Factors
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Atrial Natriuretic Factor
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DNA