A drop in neuronal firing leads to a drop in somatic calcium. This decreases the amount of activated CaMKIV (CaMKIV*) in the nucleus, and increases transcription of a “Scaling Factor” that enhances AMPA receptor accumulation at synapses through an unknown mechanism. This increases excitatory synaptic strength and raises firing rates back to target levels. There may be several signaling pathways that can act in parallel to generate synaptic scaling (dashed arrows); for example, decreased neuronal excitation will decrease the immediate early gene product Arc; reduced Arc levels increase AMPA receptor accumulation by reducing endocytosis.

Blocking network activity with tetrodotoxin reduces release of the brain-derived neurotrophic factor (BDNF) from neurons, and the cytokine tumor necrosis factor α (TNFα) from glial cells. A chronic (several days) reduction in BDNF and increase in TNFα both scale up excitatory synapses onto pyramidal neurons.

A) Correlated presynaptic and postsynaptic firing induces long-term potentiation (LTP), which then allows the presynaptic neuron to drive the postsynaptic neuron more strongly. This increases the correlation between presynaptic and postsynaptic activation, which drives more LTP, and so on in an unconstrained positive feedback cycle. B) Unconstrained LTP will lose synapse-specificity, because when one input undergoes LTP and drives the postsynaptic neuron more strongly, it makes it easier for other inputs to make the postsynaptic neuron fire, and they begin to undergo LTP as well. C) Homeostatic synaptic scaling prevents this runaway potentiation. When LTP of one input increases postsynaptic firing, synaptic scaling will reduce the strength of all synaptic inputs until the firing rate returns to control levels. Note that synaptic strengths are reduced proportionally, so that the relative strength of the potentiated synapse remains the same.

A. Blocking postsynaptic firing while leaving presynaptic and network activity intact scales up synaptic strengths in the dendrites, via a mechanism that results in increased accumulation of both GluR1 and GluR2 subunits of AMPA receptors. B. When action potential firing is blocked and NMDA receptor activation is locally blocked with the antagonist APV, there is a local increase in synaptic GluR1 accumulation that requires local dendritic protein synthesis.

A firing rate set-point can be constructed out of two opposing synaptic scaling factors. Factor+ (solid red line) increases in activation as activity rises, and scales down the strengths of excitatory synapses strengths. Factor- (solid blue line) increases in activation as activity falls, and scales up synaptic strengths. The firing rate at which these two opposing forces equalize is the activity set-point (solid black circle). If one of these factors is reduced or increased in magnitude, the activity set-point will shift: for example, if Factor+ is reduced in magnitude (dashed blue line), the activity set-point will shift to a more negative value (solid gray circle).