Send to:

Choose Destination
See comment in PubMed Commons below
Methods Mol Biol. 2008;488:129-51. doi: 10.1007/978-1-60327-475-3_9.

Characterization of RNA-protein interactions by phosphorothioate footprinting and its applications to the ribosome.

Author information

  • 1Department of Biochemistry, University of Pretoria, Pretoria, South Africa.


Analogs of naturally occurring substances obtained by chemical modifications are powerful tools to study intra- and intermolecular interactions. We have used the phosphorothioate technique to analyze RNA-protein interactions, here the interactions of transfer RNAs (tRNAs) with the three ribosomal binding sites. We describe preparation and purification of thioated tRNAs, formation of functional complexes of programmed ribosomes with tRNAs, and the evaluation of the observed phosphorothioate footprints on the tRNAs.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Springer
    Loading ...
    Write to the Help Desk