Detection of M1, NP, and M1 proteins in TIV by cytotoxic CD8+ and CD4+ T cell lines. A. The CD8+ T cell line, 1-1 specific to the NP 383-391 epitope was tested for recognition of autologous target cells infected with three TIV vaccines at concentrations from 5-.05 μg/ml at an E/T ratio =10. ISCOMATRIX was first incubated with vaccinefor 24 hours, added to autologous BLCL for 1 hour, washed and then incubated overnight at 37 °C. B. The CD8+ T cell line, 1–7K, specific to M158-66 epitope was tested for recognition of autologous target cells infected with three TIV vaccines at concentrations from 5-.05 μg/ml at an E/T ratio =10. ISCOMATRIX was first incubated with vaccinefor 24 hours, added to autologous BLCL for 1 hour, washed and then incubated overnight at 37 °C. C. The CD4+ T cell line, 3E-2 specific to HA 267-283 epitope was tested for recognition of autologous target cells infected with three TIV vaccines at concentrations from 5-.05 μg/ml at an E/T ratio =10. D. The CD4+ T cell line, 1–3, specific to M1 17-31 epitope was tested for recognition of autologous target cells infected with three TIV vaccines at concentrations from 5-.05 μg/ml at an E/T ratio =10.