Cell fate in the Arabidopsis root is determined by positional information mediated by plant hormones and interpreted by transcriptional networks. In this review, we summarize recent advances in our understanding of the regulatory networks that control cell fate within the root meristem, and in the interplay of these networks with phytohormones. Recent work describing the importance of chromatin organization in tissue patterning is also highlighted. A new, high resolution root expression map detailing the transciptome of nearly all cell types in the Arabidopsis root across developmental timepoints will provide a framework for understanding these networks.